目的 研究肉桂精油对正常大鼠肠道梭菌属IV簇菌群和拟杆菌属两类主要菌群的影响。方法 SD大鼠每天给予肉桂精油0.384 3 g/kg，连续给药30 d，取结肠和直肠内容物，针对肠道菌群的16S rRNA，采用末端限制性片段多态性技术（TRFLP）分析肠道梭菌属IV簇细菌和拟杆菌属细菌的数量、多样性及其分布。结果 肉桂精油给药后，肠道梭菌IV簇细菌和拟杆菌的数量、多样性及分布均发生显著变化，尤其是肠道梭菌IV簇细菌的变化更大。肉桂精油对结肠和直肠中两类菌的影响存在差异。结论 肉桂精油对肠道梭菌IV簇和拟杆菌属两类主要菌群的影响可能是肉桂对肥胖和糖尿病的作用机制之一。

Objective To investigate the impact of essential oil (cinnamon oil) from Cinnamomum cassia (EOCC) on Clostridia flora IV and Bacteroides in gut of normal rats. Methods SD rats were administered with EOCC (0.384 3 g/kg) for 30 d and the colon and rectal contents were sampled. The population, diversity, and distribution of Clostridium flora IV and Bacteroides in the samples were analyzed by the terminal restriction fragment length polymorphism (TRFLP) method based on the intestinal flora 16S ribosomal RNA gene. Results Both the intestinal flora population, diversity, and distribution of Clostridia flora IV and Bacteroides changed significantly due to the injection, especially for Clostridia flora IV. Additionally, EOCC exerted the different effects on colon and rectal microflora. Conclusion The effects of EOCC on Clostridia flora IV and Bacteroides in gut might be the functional mechanism of cinnamon oil in curing obesity and diabetes.

中央高校基本科研业务费专项基金资助项目（21611414）