目的探讨枸杞多糖对小鼠移植性肝癌的抑制作用。方法采用皮下接种法制备小鼠移植性肝癌(H22肝癌细胞株)模型,随机将96只小鼠分成对照组、模型组、枸杞多糖高、中、低剂量(20、10、5 mg/kg)组、环磷酰胺(20 mg/kg)组。观察枸杞多糖对荷瘤小鼠死亡率、肿瘤体积、肿瘤质量及肿瘤细胞密度、核分裂像、肿瘤坏死程度及间质淋巴细胞浸润、纤维组织增生情况的影响。结果 20、10、5 mg/kg的枸杞多糖能明显降低移植性肝癌小鼠的死亡率(P<0.05),减小肿瘤细胞的体积和质量(P<0.05),抑瘤率分别为42.23%、25.10%和9.16%;同时20、10 mg/kg的枸杞多糖能不同程度地降低肿瘤细胞密度(P<0.05),减少肿瘤细胞的核分裂像计数(P<0.05),增加肿瘤细胞坏死程度、间质淋巴细胞浸润和纤维组织增生(P<0.05)。结论枸杞多糖对小鼠移植性肝癌有一定的抑制作用。

Objective To explore the inhibition of Lycium barbarum polysaccharide (LBP) on transplanted liver cancer in mice. MethodsThe transplanted liver cancer model was established with hypodermic inoculation. Ninety-six mice were randomly divided into six groups: control group, model group, LBP high-, middle-, and low-dose (20, 10, and 5 mg/kg) groups, and cyclophosphamide (20 mg/kg) group. The effects of LBP on mortality, gross tumor volume, inhibitory rate of tumor growth, tumorous cellular density, karyokinesis, degree of tumor necrosis, lymphocyte infiltration, and fibroplasia in interstitial substance in tumor-bearing mice were observed. Results LBP with high, middle, and low doses could obviously depress the death rate of tumor-bearing mice (P < 0.05) and diminish gross tumor volume and weight (P< 0.05). The inhibitory rates of tumor growth were 42.23%, 25.10%, and 9.16%. LBP with high and middle doses could degrade the tumorous cellular density (P< 0.05), decrease the karyokinesis (P < 0.05), and increase the degree of tumor necrosis, lymphocyte infiltration, and fibroplasia in interstitial substance in mice (P < 0.05). Conclusion LBP could produce the inhibition on transplanted liver cancer in mice.

宁夏自然科学基金项目(NZ1092);宁夏医科大学科研项目(XM2011002)