目的研究制川乌配伍甘草对制川乌中多种单酯型生物碱煎出量的影响,探索其变化规律,以指导制川乌与甘草的合理配伍。方法 HPLC法测定制川乌与甘草不同比例配伍时煎出液中单酯型生物碱的总量,并通过对比,分析不同配比对煎出量的影响。结果随着甘草配伍量的增加,煎煮液中单酯型生物碱总量逐渐降低,每种单酯型生物碱煎出量趋于单煎液煎出量的40%。结论甘草配伍制川乌时,甘草的使用量应是制川乌的一半以上,既可以保证煎出液中单酯型生物碱总量稳定可控,又可以防止制川乌毒性过大造成使用危险。

Objective To study the effect of Aconiti Radix Preparata (RAP) combined with Glycyrrhizae Radix (RG) in different ratios on the decocted yield of monoester-type alkaloid from RAP and to investigate the disciplines of various compatibilities. Methods HPLC was adopted to determine the monoester-type alkaloid in decoction with different ratios of RAP and RG. The influence of the various compatibilities on alkaloid was analyzed.Results The total decocted yields of monoester-type alkaloid in the decoction decreased with the increasing of RG in the decoction. The monoester-type alkaloid in compatibility tended to be 20% of RAP single decoction. Conclusion The usage of RG should be more than half of that of RAP when using together, which could make the yields of monoester-type alkaloid stable and controllable and avoid the danger for use from the excessive toxicity of RAP.

“十一五”国家重大新药创制课题(2010ZX09401-406)