目的 运用组织培养技术诱导平贝母四倍体。方法 采用不同质量浓度（200、500、1 000、2 000 mg/L）秋水仙素和不同的诱导时间（12～72 h）处理平贝母愈伤组织。结果 离体培养条件下，平贝母激素自养型培养材料在秋水仙素诱导下得到四倍体，四倍体染色体4n＝44，四倍体植株出现明显的多倍体特征。结论 500 mg/L秋水仙素处理24 h为最佳组合，可以诱导四倍体平贝母以平贝母子球做鉴定材料，获得较好的结果。

Objective To induce the tetraploid of Fritillaria ussuriensis via tissue culture. Methods F. ussuriensis calli were treated with colchicine at different concentration and time periods. Results Tetraploid was obtained by F. ussuriensis hormone autotrophic cultural material induced by colchicine in vitro. The chromosome number of tetraploid was 4n = 44. Conclusion The best combination is 500 mg/L colchicine treated for 24 h and better result is obtained by using the bulb of F. ussuriensis as testing materials.

吉林省财政厅项目；长春净月开发区项目（2009c005）