目的探讨半夏毒针晶引起的刺激性炎症的机体表现及对相关炎症介质的影响。方法采用小鼠毛细管通透性实验、小鼠腹腔炎症实验及大鼠足跖肿胀实验,考察不同剂量半夏毒针晶的致炎效应及其量-效关系,并测定相关炎症介质的量。结果半夏毒针晶混悬液可使小鼠腹腔毛细血管通透性增加,腹腔渗出液中炎症介质前列腺素E₂(PGE₂)、一氧化氮(NO)、丙二醛(MDA)的量增加;亦可引起大鼠足跖肿胀,并在一定剂量范围内表现为典型的量-效关系,还可使大鼠致炎足跖中炎症介质PGE₂、环氧合酶-2(COX-2)的量显著增加。结论半夏毒针晶刺激性毒性在机体内表现为炎症反应。

Objective To study the irritating inflammation and the influence on contents of inflammatory mediators induced by the toxic raphides from Pinellia ternata. Methods The mouse capillary permeability experiment, models of peritoneal inflammation in mice and toe swelling in rats were applied to investigating the inflammatory effects of the toxic raphides from P. ternata at different concentration. And the content of inflammatory mediators was detected. Results The results showed that the suspension of the toxic raphides from P. ternata could enhance the capillary permeability and make the release of prostaglandin E₂(PGE₂), nitric oxide (NO),and malondialdehyde (MDA) in peritoneal exudate increased in a dose-dependent manner. The toxic raphides from P. ternata also could cause toe swelling in rats and significantly increase the concentration of PGE₂ and cyclooxygenase (COX-2) in toes of rats,which showed a typical dose-response relationship in a certain dose range. Conclusion The toxic raphides from P. ternata could cause severe inflammatory toxicity in vivo.

国家自然科学基金资助项目(30772785;30973939;1173549);“十一五”国家科技重大专项课题(2009ZX09308-004)