目的研究乙酰氧基胡椒酚乙酸酯(ACA)亚微乳和ACA原料药对HeLa细胞生长和凋亡的影响。方法用5、12.5、25、50、100、200μmol/L ACA亚微乳和ACA原料药处理HeLa细胞,MTT比色法检测ACA亚微乳和原料药作用24、48、72、96 h对HeLa细胞增殖的抑制作用;倒置显微镜观察HeLa细胞形态改变;流式细胞仪检测细胞凋亡和细胞周期。结果 ACA亚微乳和原料药均可明显抑制HeLa细胞增殖,其中ACA亚微乳的抑制效果较好;经ACA亚微乳和原料药处理的HeLa细胞显示典型的凋亡形态改变;HeLa细胞凋亡率与ACA处理时间、浓度呈正相关;ACA亚微乳和ACA原料药均使HeLa细胞阻滞于S期,且ACA亚微乳作用强于ACA原料药。结论与ACA原料药相比,ACA亚微乳抑制HeLa细胞增殖和诱导凋亡的作用更显著。

Objective To investigate the effects of 1′-acetoxychavicol acetate (ACA) submicron emulsion and its drug substance on HeLa cells. Methods Human HeLa cell was cultured and treated with 5, 12.5, 25, 50, 100, and 200 μmol/L ACA submicron emulsion and its drug substance, respectively. MTT was used to detect the inhibition of proliferation in 24, 48, 72, and 96 h. Inverted microscope was performed to observe the morphologic changes of HeLa cells. Flow cytometry was used to evaluate apoptosis and cell cycle. Results ACA submicron emulsion and its drug substance could significantly inhibit the proliferation of HeLa cells. Cells cultured with ACA submicron emulsion and its drug substance were observed to present typical apoptosis and cell cycle changes. Flow cytometry revealed a time- and dose-dependent relationship. Conclusion Both ACA submicron emulsion and its drug substance could inhibit the proliferation of HeLa cells, and the inhibition of ACA submicron emulsion is better.

河南省重点科技攻关计划项目(112102310022);河南省预研专项基金项目(10yy0036)