目的针对延胡索抑制肝癌细胞的作用,结合正交设计法,优选延胡索中延胡索乙素、原阿片碱的提取工艺条件,并对2种生物碱与药效学指标的相关性进行分析。方法采用正交设计法,以延胡索乙素提取率、原阿片碱提取率、SMMC-7721细胞抑制率和出膏率为评价指标,对延胡索中2种生物碱的提取工艺条件进行优选;应用SPSS 13.0统计软件进行Pearson相关系数分析。结果最佳提取工艺为6倍量70%乙醇回流提取2 h,延胡索中2种生物碱的提取率最高,对肝癌细胞的抑制率最高。结论该方法快速、操作简便,可以为确定延胡索中2种生物碱定量测定方法提供实验依据。

Objective To evaluate the inhibitory effect of Corydalis Rhizoma on hepatoma carcinoma cell, the extraction process of two alkaloids in Corydalis Rhizoma was optimized with orthogonal design and the coefficient correlation for the two alkaloids and pharmacodynamic index was analyzed. Methods The extraction process of the two alkaloids was optimized using orthogonal design with four indexes which included the extraction rates of tetrahydropulmatine and biflorine, MTT inhibitory ratio of SMMC-7721 cell,and the rate of extractum extracted. Pearson correlations were used for analysis on coefficient correlation with SPSS 13.0 software. Results The optimal conditions were to reflux extraction with 70% alcohol as much as six times of the medicine amount for once in 2 h.The highest contents for both tetrahydropulmatine and biflorine and the highest inhibitory rate of SMMC-7721 cell were demonstrated. Conclusion The rapid and simple method could provide the experimental support for the quantitative determination of two alkaloids in Corydalis Rhizoma.

“十一五”重大新药创制科技重大专项“组分中药配伍研究技术子平台”(2010ZX09401-304-105C)