目的研究银杏叶提取物用于衰老大鼠模型的血清指纹图谱,探讨其体内药效成分。方法建立D-半乳糖致衰老大鼠模型,同时ig给予银杏叶提取物,制备衰老大鼠的含药血清,采用HPLC-UV和HPLC-ESI-MS法,分别针对黄酮类成分和内酯类成分建立指纹图谱,分析入血成分。结果 HPLC-UV指纹图谱显示了10个入血成分,大多为代谢成分;HPLC-MS指纹图谱标定了9个共有峰,其中7个为原型成分。结论首次进行了银杏叶提取物的血清药物成分图谱研究,可反映银杏叶提取物口服给药吸收入血药物成分的变化,为其体内药效物质研究奠定基础。

Objective To investigate the serum fingerprint of extract from Ginkgo biloba leaves (EGBL) in aging rats and explore the pharmacological constituents in vivo. Methods D-galactose was used to induce aging rats, then rat serum was prepared after ig administration of EGBL. Aimed at flavonoids and terpenoids, HPLC-UV and HPLC-ESI-MS methods were developed, respectively,and chemical constituents in blood were investigated. Results As a result, HPLC-UV fingerprint showed ten common peaks in serumof rats and most of them were metabolite. In HPLC-MS fingerprint, there were nine common peaks among which seven peaks were original compounds. Conclusion This study explores the fingerprint of EGBL in aging rat serum for the first time, it can reveal the change of chemical constituents after ingestion, and provide some data on material basis study in vivo for EGBL.

湖南省教育厅研究项目(09C629);湖南省中医药科研计划项目(2010082);2009年湖南省大学生创新性实验计划项目