目的研究尖山橙Melodinus fusiformis枝叶非生物碱部位的化学成分。方法采用柱色谱进行分离,通过理化性质和光谱数据鉴定结构。结果从尖山橙枝叶甲醇提取物的醋酸乙酯部位分离得到28个化合物,分别鉴定为oleanderolide(1)、麦珠子酸(2)、3β-acetoxylup-20(29)-ene(3)、11,12-去氢乌索酸内酯(4)、齐墩果内酯(5)、24R-乙基-5α-胆甾烷-3β,6α-二醇(6)、(+)-松脂酚(7)、8α-羟基松脂酚(8)、番木鳖苷A(9)、紫云英苷(10)、α-tocopherol(11)、butyl isobutyl phthalate(12)、11-羟基柳叶水甘草碱(13)、(+)-voaphylline(14)、丁香树脂酚(15)、(+)-1-羟基丁香树脂酚(16)、(+)-fraxiresinol(17)、1-(4-hydroxy-3,5-dimethoxyphenyl)-hexahydro-1H-cyclopentafuran-4-o(l 18)、(±)-acyloxy enone(19)、2-羟基苯甲酸(20)、邻苯二甲酸二丁酯-N,N-二乙基-2-羟基苯甲酰胺(21)、6-羟基吲哚(22)、1,3-二油酸甘油酯(23)、邻苯二甲酸二丁酯(24)、双(2-乙基丁基)对苯二甲酸酯(25)、(6Z,8E,17E)-icosa-6,8,17-trien-lo-ol(26)、β-谷甾醇(27)、乌索酸(28)。结论尖山橙化学成分复杂多样,所分离的成分中有26个化合物(1～12、15～28)为首次从该植物中获得。

Objective To study the chemical constituents from non-alkaloid fraction in the extract from twigs and leaves of Melodinus fusiformis. Methods The chemical constituents were separated and purified with chromatographic techniques and their structures were elucidated on the basis of spectral analysis, as well as the chemical properties. Results A total of 28 compounds were obtained from ethyl acetate fraction of the methanolic extract from twigs and leaves of M. fusiformis and were identified as oleanderolide (1),alphitolic acid (2), 3β-acetoxylup-20(29)-ene (3), 11, 12-dehydroursolic acid lactone (4), oleanolic lactone (5), 24R-ethyl-5α-cholestane-3β, 6α-diol (6), (+)-pinoresinol (7), 8α-hydroxypinoresinol (8), loganin A (9), astragalin (10), α-tocopherol (11), butyl isobutyl phthalate (12), 11-hydroxytabersonine (13), (+)-voaphylline (14), syringaresinol (15), (+)-1-hydroxysyringaresinol (16),(+)-fraxiresinol (17), 1-(4-hydroxy-3, 5-dimethoxyphenyl) hexahydro-1H-cyclopentafuran-4-ol (18), (±)-acyloxy enone (19),2-hydroxybenzoic acid (20), N, N-diethyl-2-hydroxybenzamide (21), 6-hydroxyindol (22), 1, 3-diolein (23), dibutyl phthalate (24),bis-(2-ethylhexyl)-terephthalate (25), (6Z, 8E, 17E)-icosa-6, 8, 17-trien-lo-ol (26), β-sitosterol (27), and ursolic acid (28). Conclusion The types of chemical composition in M. fusiformis are complex and diverse. Twenty-six compounds (1—12 and 15—28) reported in this study are obtained from this plant for the first time.