目的 研究薏苡仁油（康莱特注射液）体外对人原位胰腺癌BxPC-3细胞生长和血管生长因子的影响，探讨薏苡仁油抗肿瘤作用机制。方法 薏苡仁油作用BxPC-3细胞后，经瑞氏染色观察细胞形态，Hoechst 33258荧光染色、DNA梯度电泳观察细胞凋亡；流式细胞仪检测细胞周期的变化；ELISA法检测血管内皮生长因子（VEGF）、成纤维细胞生长因子（bFGF）的变化。结果 薏苡仁油（2 mg/mL，20 μL/mL）作用于BxPC-3细胞后，瑞氏染色可见细胞出现典型的凋亡小体，Hoechst 33258荧光染色可见BxPC-3细胞出现特征性凋亡变化，琼脂糖电泳观察到明显的细胞凋亡特征性DNA梯形条带；细胞周期阻滞在G₀/G₁期；细胞上清液中VEGF、bFGF的表达水平明显下调。结论 薏苡仁油能影响BxPC-3细胞生长周期，导致细胞周期阻滞，下调VEGF和bFGF的表达水平，可能对抑制胰腺癌细胞的扩散产生一定作用。

Objective To investigate the effect of Coicis Semen oil (Kanglaite Injection) on the proliferation, apoptosis, and vascular endothelial growth factor (VEGF) of human in situ pancreatic cancer cell line BxPC-3 in vitro, and to explore its mechanism. Methods BxPC-3 cells in vitro were treated with Coicis Semen oil (2 mg/mL, 20 μL/mL), cell morphology was observed by Wright’s staining; Apoptosis was assayed by Hoechst 33258 fluorescence staining and DNA gredient agarose gel electrophoresis; Diversification of cell cycle was detected by flow cytomletry; Changes of VEGF and fibroblast growth factor (bFGF) were detected by ELISA. Results Wright’s staining showed characteristic apoptotic body in BxPC-3 cells; Hoechst 33258 fluorescence staining showed characteristic change of apoptosis; Obvious DNA-ladder strap was observed by agarose gel electrophoresis; Cell cycle was retarded on G₀/G₁ stages; The level of VEGF and bFGF expression in the cell liquid supernatant has been a distinct decline. Conclusion Coicis Semen oil could affect the cell growth cycle of BxPC-3 cells, conduce the retardance of cell cycle, and reduce the level of VEGF and bFGF expression, which may help for restraining the pervasion of pancreatic cancer cells to some extent.

浙江省自然科学基金资助项目（Y2080506）；浙江省教育厅重点项目（Z200908281）；浙江省中医药管理局资助项目（2010ZB026）；浙江省大学生创新创业孵化项目（2010R410059）