目的 比较以半仿生提取法(SBE法)、醇提取法(AE法)、水提取法(WE法)制得黄连解毒汤方药提取液的指纹图谱,进一步验证该方药较佳的提取方法。方法 采用反相高效液相色谱(RP-HPLC)法,比较3种方法制备的黄连解毒汤相对分子质量≤1 000的提取液的指纹图谱。结果 SBE提取液有23个特征指纹峰;与SBE提取液相比较,AE提取液缺少5个特征指纹峰(5、9、10、11、22),WE提取液缺少3个特征指纹峰(11、22、23);用夹角余弦法计算特征指纹峰的相似度,结果 SBE提取液的数值均大于AE提取液和WE提取液,这与共有特征峰重叠率的计算结果也较相符。栀子苷、黄芩素、盐酸小檗碱3种指标成分及共有特征指纹峰总面积比较,皆是SBE提取液>AE提取液>WE提取液。结论 黄连解毒汤方药以SBE法提取得到的相对分子质量≤1 000的提取液中成分最多。

Objective To compare HPLC fingerprints of extract solutions from Huanglian Jiedu Decoction (HJD) made bysemi-bionic extraction (SBE), alcohol extraction (AE), and water extraction (WE), and verify the best extraction method furtherly.Methods Fingerprint of extract solutions with relative molecular mass (RMM)≤1 000 from HJD made by the three differentmethods was investigated and compared by RP-HPLC. Results There were 23 characteristic peaks in SBE solution, while there werefive characteristic peaks (5, 9, 10, 11, 22) in AE solution and three (11, 22, 23) in WE solution less than those in SBE solution. Datawere analyzed by fingerprint similarity evaluation software and the similarity of characteristic peaks in SBE solution was larger thanthose in AE and WE solutions, which was in accordance with the overlap rate of common peak. The comparion between geniposide,baicalein, and berberine and the total area of characteristic peak is SBE＞AE＞WE, respectively. Conclusion The optimal methodfor making the extract solution with RMM≤1 000 of HJD is SBE.

国家科技重大新药创制专项——“中药‘半仿生'提取关键技术研究”(2010ZX09401)