目的: 优选白花刺参的最佳回流提取工艺条件。方法: 采用UV法和HPLC法测定总皂苷及刺参苷J的量, 以总皂苷提取率、刺参苷J提取率和干浸膏收率为综合评价指标, 采用U₈(8⁴)均匀设计表设计试验, 考察乙醇体积分数、乙醇用量、提取次数、提取时间4种因素对总皂苷提取的影响。结果: 白花刺参的最佳提取工艺参数: 80%乙醇回流提取2次, 每次1h。结论: 均匀设计法优选出的白花刺参总皂苷的提取工艺合理可行, 为白花刺参总皂苷的进一步研究提供参考。

Objective: To optimize the extractive condition of Morina nepalensis by hot refluxing. Methods: HPLC and UV were established to determine the monepaloside J and total saponins. U₈(8⁴) Uniform design form was selected as the experiments’schedule, the total saponins, monepaloside J and the yield was used as evaluation indicator to and the ethanol concentration, amount of extraction solvent added, extractive time and times of extraction was used as the 4 influencing factors. Results: The optimum condition was the extraction in 80% ethanol, two times and 1h. Conclusion: The optimal extracting method of the total saponins of M.nepalensis is suitable and feasible by uniform design. The study could provide scientific references for further investigation of M.nepalensis.

四川省科技厅应用基础研究项目(2009JY0017);西南民族大学中央高校基本科研业务费专项资金资助(09NZYZJ01)