目的 对党参肌动蛋白（actin）基因进行克隆及序列分析。方法 根据已经克隆的植物actin基因的保守序列设计一对简并性引物，以党参根部总RNA为模板，采用RT-PCR的方法扩增actin 基因片段并连接到pMD19-T Simple载体上，阳性克隆经PCR检测后进行测序。结果 得到一段603 bp的序列，序列分析表明，该片段编码200个氨基酸，与高等植物actin基因核苷酸序列同源性在78%以上，与其他肌动蛋白氨基酸序列同源性达90%以上。结论 首次从党参中克隆出了actin基因，为有效利用该基因奠定了基础。

Objective To clone and sequence the cDNA encoding actin gene from the roots of Codonopsis pilosula. Methods Degenerate primers were designed based on the conserved sequences of the actin gene from other plants. Total RNA was extracted from the roots of C. pilosula. Actin gene fragment was obtained by reverse transcription polymerase chain reaction (RT-PCR) and PCR products are sub-cloned into pMD19-T Simple vector. The positive clone identified by PCR was sequenced. Results The sequencing result revealed that the actin gene fragment from the roots of C. pilosula contained about 603 bp, encoding 200 amino acids. Sequence analysis suggested that the nucleotide sequence and the translated amino acid sequence shared over 78% and 90% of homology respectively with actin gene sequences from other higher plants. Conclusion It is the first report that a novel actin gene is cloned from roots of C. pilosula.This work lays a foundation for application to actin gene.

甘肃省中医药科研立项课题（GZK-2010-41）；兰州市科技发展计划项目（2010-1-39）；教育部新世纪优秀人才支持计划；中央高校基本科研业务费专项资金项目（LZUJBKY-2010-2）