目的优选左金丸最佳提取方法。方法采用超高效液相色谱法(UPLC),建立左金丸的指纹图谱以及5种生物碱的测定方法;以指纹图谱相似度和5种生物碱的总量作为评价指标,对左金丸的水提、70%乙醇提取、盐酸-甲醇(1:100)提取、半仿生提取方法进行比较。结果左金丸的色谱峰在8 min内检测完,共检测到9个共有峰,基线平稳,峰形较好。5种生物碱的线性关系良好,分离度高;不同提取方法的左金丸指纹图谱相似度>0.98;而5种生物碱的量差异较大,其中盐酸-甲醇(1:100)提取的左金丸中5种生物碱的总量最高,水提方法最低。结论本实验建立的方法操作简便,分析速度快;基于指纹图谱的定性判别和指标成分的定量分析,可以综合全面地评价比较左金丸的提取方法;其中盐酸-甲醇(1:100)提取左金丸的方法优于其他3种方法。

To study Zuojin Pills with different extraction methods and select the best one. Methods To establish the fingerprints and determination the content of five alkaloids in Zuojin Pills by ultra performance liquid chromatography (UPLC), compare the water extraction, 70% ethanol extraction, HCl-methanol (1:100) extraction, and semi-bionic extraction according to the similarity of fingerprints and content of five alkaloids. Results The appearance time of all chromatographic peaks of Zuojin Pills was within 8 min and 9 common chromatographic peaks were obtained. The linear is stable and peak form is better. The linear relationship and resolution of the five alkaloids are nice. The similarity of the fingerprints exceed 0.98; The contents of the five alkaloids are much more different, the content of the five alkaloids in the HCl-methanol sample is the most, however, the least is the water extraction sample. Conclusion This study shows that the UPLC method is convenient with quick analysis rate. On account of indicated component quantitative analysis, it could be used to synthetically evaluate the extraction method. HCl-methanol (1:100) extraction method is the best one.

国家重点基础研究发展计划(“973”计划)(2007CB512607);国家自然科学基金资助项目(30772740);国家科技重大专项课题(2009ZX10005-017)