目的 对影响翼梗五味子ISSR-PCR扩增反应的各因素进行优化，建立稳定的反应体系。方法 基于正交极差分析方法，以Taq酶、Mg²⁺、模板DNA、dNTP和引物5因素4水平的正交试验对翼梗五味子ISSR-PCR反应体系进行优化。结果 翼梗五味子ISSR-PCR的最佳反应体系（20 μL）为：Taq酶1.00 U、Mg²⁺ 1.50 mmol/L、模板DNA 40.00 ng、dNTP 0.25 mmol/L、引物0.50 μmol/L。筛选出12条扩增稳定、多态性丰富的ISSR引物，并确定了每个引物的最佳退火温度。结论 所建立的翼梗五味子ISSR反应体系具有标记位点清晰、反应系统稳定、重复性好等优点，为翼梗五味子的分子水平研究提供实验依据。

Objective To optimize the each factor affecting on ISSR-PCR reaction system of Schisandra henryi and establish the stable ISSR-PCR system. Methods Based on the analysis of orthogonal design test, an orthogonal design was used to optimize the ISSR-PCR amplification system on S. henryi by five factors (Taq polymerase, Mg²⁺, DNA template, dNTP, and primer) at four concentration levels, respectively. Results A suitable ISSR-PCR reaction system was constructed with the 20 μL reaction system containing 1.00 U Taq polymerase, Mg²⁺1.50 mmol/L, DNA template 40.00 ng, dNTP 0.25 mmol/L, and primer 0.50 μmol/L. Twelve effective ISSR primers were selected and the optional annealing temperature of every one primers was fixed. Conclusion ISSR-PCR is significantly influenced by the concentration of S. henryi. This ISSR-PCR system could provide clear bands, reliable reaction system, and abundant polymorphisms. It proves a reference for molecular research of S. henryi.

国家自然科学基金资助项目（31070293）；国家“十一五”科技支撑计划项目（2006BAI06A13-06）