目的 研究滇重楼种子破休眠的方法及其内源激素水平变化规律。方法 以变温层积处理的滇重楼种子为材料,利用高效液相色谱法(HPLC)测定种胚发育过程中植物激素赤霉素(GA)、吲哚乙酸(IAA)、脱落酸(ABA)量的变化与种子胚率的关系。结果 变温层积处理可在3个月内打破滇重楼种子休眠,其GA量显著升高,ABA量显著下降。结论 内源激素ABA和GA在滇重楼种子休眠与萌发过程中起着重要的作用。

Objective To study the rule of breaking seed dormancy and content changes of endogenous hormone of the Paris polyphylla var. Yunnanensis seeds. Methods The seeds were treated under fluctuating temperature, and the content of endo-hormone,such as GA, IAA, and ABA, was mensurated by HPLC. Results During the first three months, the stratification under fluctuating temperature could break the seed dormancy. The content of GA rose obviously and the content of ABA reduced. Conclusion It is suggested that ABA and GA have significant effect on the dormancy and germination of P. polyphylla var. yunnanensis seeds.

国家自然科学基金资助项目(30860367)