目的 观察终末糖基化产物(AGEs)对小鼠神经小胶质细胞BV-2炎症因子白细胞介素(IL-1β、IL-6)表达的影响及天麻素的干预效应。方法 将体外培养的小鼠小胶质细胞分成对照组、AGEs组、天麻素(12.5、25、50、100 mg/L)组,培养18 h,观察细胞形态变化,酶联免疫吸附法(ELISA)测定培养上清中IL-1βI、l-6水平,逆转录聚合酶链反应(RT-PCR)方法测定IL-1β、IL-6 mRNA表达。结果 倒置显微镜下观察,对照组小胶质细胞多为静止期,突起较少,AGEs组多数细胞突起较多,呈阿米巴样,以AGEs质量浓度为300 mg/L时最明显,天麻素干预组细胞突起较AGEs组有所减少,其中以天麻素50 mg/L组最少。与对照组相比,AGEs组细胞培养上清液中IL-1β、IL-6水平,以及细胞内IL-1β、IL-6 mRNA表达明显增加,具有显著差异(P<0.05);与AGEs组相比,天麻素组细胞培养上清液中IL-1β、IL-6水平,以及细胞IL-1β、IL-6 mRNA表达均有所降低,其中天麻素25、50、100 mg/L组差异显著(P<0.05),以天麻素50 mg/L组降低最显著(P<0.01)。结论 AGEs能够诱发神经小胶质细胞产生IL-1β、IL-6,天麻素能部分抑制AGEs对小胶质细胞的诱导作用。

Objective To investigate the effects of gastrodine (GAS) on the expression of IL-1β and IL-6 in nerve microglia BV-2 cell of mice induced by different concentrations of advanced glycation end products (AGEs) and GAS intervention. Methods The cultured BV-2 cells of mice were divided into AGEs group, GAS (12.5, 25, 50, and 100 mg/L) groups, which were cultured for 18 h to observe the changes of cell morphology. The levels of IL-1β and IL-6 in the cell culture supernate were determined by enzyme linked immunosorbent assay (ELISA) and the expressions of IL-1β and mRNA were analysed by reverse transcriptase polymerase chain reaction (RT-PCR). Results Observed under an inverted microscope, microglia is mostly quiescent and less protruding in control group; Most cell processes were more and amoeba-like in AGEs group, which was most obviously at the concentration of 300 mg/L. Cell processes in GAS groups reduced less than those in AGEs groups, of which the least was in GAS group at concentration of 50 mg/L. Compared with the control group, the levels of IL-1β and IL-6 in supernatants of cell culture in AGEs group and the expressions of IL-1β and IL-6 mRNA increased with significant difference (P<0.05). Compared with AGEs groups, the levels of IL-1β and IL-6 in supernatants of cell culture in AGEs group and the expressions of IL-1β and IL-6 mRNA obviously decreased, there were significant differences in AGE groups at the concentrations of 25, 50, and 100 mg/L (P<0.05), among which the decrease was most significant in the AGE group of 50 mg/L (P<0.01). Conclusion AGEs could induce that nerve microglia produce IL-1β and IL-6 and GAS could partly inhibit the induction of AGEs on microglia.

温州市科技局基金资助课题(Y20070010)