目的 探讨蚕蛹油对高脂血症大鼠的调血脂作用及机制。方法 高脂饮食复制大鼠高脂血症模型,以蚕蛹油ig给药6周,每两周检测大鼠血清中甘油三酯(TG)、总胆固醇(TC)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)、载脂蛋白AI(ApoAI)、载脂蛋白B(ApoB)、卵磷脂胆固醇转移酶(LCAT)、脂蛋白脂酶(LPL)、肝脂酶(HL)水平,第6周处死后,称量肝质量,并检测肝中LPL、HL水平。结果 蚕蛹油各组的血脂(TG、TC)、脂蛋白(HDL-C、LDL-C)、载脂蛋白(ApoAI、ApoB)、LCAT及总酯酶水平与模型组各指标相比均具有显著差异(P<0.05),并具有剂量依赖性,且相应酶的变化早于血脂和脂蛋白的变化。结论 蚕蛹油可以通过激活载脂蛋白和脂代谢相关酶,调整血清中血脂和脂蛋白水平,从而达到治疗高脂血症的目的。

Objective To investigate the regulation of the silkworm chrysalis oil on the plasma lipid in hyperlipidemia rats and its mechanism. Methods The rat model of hyperlipidemia was built by high fat diet and then ig administration of silkworm chrysalis oil for six weeks to observe TG, TC, HDL-C, LDL-C, ApoAI, ApoB, LCAT, LPL, and HL levels in the serum of rat every two weeks, then killed the rats in the sixth week, weighed the liver, and detected the LPL and HL levels in the liver. Results Compared to the indexes of model group, the blood lipids (TG and TC), lipoprotein (HDL-C and LDL-C), apoprotein (ApoAI and ApoB), LCAT, LPL, and HL levels of the rats in every group treated with silkworm oil were significantly different (P＜0.05) in a dose-dependent manner, and the change of the relative enzyme level was earlier than that of the blood lipid and lipoprotein level. Conclusion The silkworm chrysalis oil could regulate the plasma lipid and lipoprotein levels in the serum of rats by activating apoprotein and correlating lipid-metabolizedenzyme, thereby to treat the hyperlipidemia.

杭州市科技局科技创新项目(2006831H05)