目的 研究玉簪Hosta plantaginea的抗炎活性部位及其化学成分。方法 萃取法制备不同极性部位,醋酸致小鼠腹腔毛细血管通透性法、棉球致小鼠肉芽肿法筛选抗炎活性部位;硅胶柱色谱等方法对活性部位进行分离纯化,根据理化性质和波谱数据进行结构鉴定。结果 醋酸乙酯部位能明显抑制小鼠腹腔毛细血管通透性增高以及棉球肉芽肿;从醋酸乙酯部位分离并鉴定了10个化合物,分别为二十二烷醇(1)、β-谷甾醇(2)、豆甾醇(3)、(25R)-2α,3β-二羟基-5α-螺旋甾烷-9(11)-烯-12-酮(4)、胡萝卜苷(5)、(25R)-2α,3β-二羟基-5α-螺旋甾烷-9(11)-烯-12-酮3-O-{O-β-D-吡喃葡萄糖基-(1→2)-O-[β-D-吡喃木糖基-(1→3)]-O-β-D-吡喃葡萄糖基-(1→4)-β-D-吡喃半乳糖苷}(6)、山柰酚3-O-(2″-O-β-D-吡喃葡萄糖基)-β-D-芸香糖苷(7)、山柰酚3-O-β-D-芸香糖苷-7-O-β-D-吡喃葡萄糖苷(8)、(25R)-2α,3β,12β-三羟基-5α-螺旋甾烷3-O-[O-α-L-吡喃鼠李糖基-(1→2)-β-D-吡喃半乳糖苷](9)、(25R)-2α,3β-二羟基-5α-螺旋甾烷3-O-[O-β-D-吡喃葡萄糖基-(1→4)-β-D-吡喃半乳糖苷](10)。结论 醋酸乙酯部位为玉簪抗炎的活性部位;化合物1～10均为首次从玉簪中分离得到,其中化合物10为新的天然产物。

Objective To screen the active fraction with anti-inflammatory effect of Hosta plantaginea and study the chemical constituents of the active fraction.Methods Different polar fractions were prepared by extraction with organic solvents.Their anti-inflammatory effects were evaluated by mice models treated by acetic acid-induced celiac capillary permeability and cotton pellets-induced granuloma.The constituents of active fraction were purified by chromatographic methods and identified by physicochemical properties and spectral data.Results The ethyl acetate fraction could significantly inhibit the reinforce of celiac capillary permeability and the cotton pellets granuloma.Ten compounds were isolated from the ethyl acetate fraction and identified as docosanol(1),β-sitosterol(2),stigmasterol(3),(25R)-2α,3β-dihydroxy-5α-spirostane-9(11)-en-12-one(4),daucosterol(5),(25R)-2α,3β-dihydroxy-5α-spirostane-9(11)-en-12-one-3-O-{O-β-D-glucopyranosyl-(1→2)-O-[β-D-xylopyranosyl-(1→3)]-O-β-D-glucopyranosyl-(1→4)-β-D-galactopyranoside}(6),kaempferol 3-O-(2″-O-β-D-glucopyranosyl)-β-D-rutinoside(7),kaempferol 3-O-β-D-rutinoside-7-O-β-D-glucopyranoside(8),(25R)-2α,3β,12β-trihydroxy-5α-spirostane 3-O-{O-α-L-rhamnopyranosyl-(1→2)-β-D-galactopyranoside}(9),and(25R)-2α,3β-dihydroxy-5α-spirostane 3-O-{O-β-D-glucopyranosyl-(1→4)-β-D-galactopyranoside}(10).Conclusion The ethyl acetate extract was the anti-inflammatory fraction of H.plantaginea.Compounds 1-10 were isolated from H.plantaginea for the first time,and compound 10 is a new natural product.