目的 探讨姜黄属Curcuma L. 6种郁金类药用植物的种间及其种内不同居群间的亲缘关系。方法 采用聚丙烯酰胺凝胶电泳（PAGE），分析超氧化物歧化酶（SOD）、多酚氧化酶（PPO）、细胞色素氧化酶（COD）的酶带，并采用UPGMA法对数据进行分析。结果 SOD、PPO和COD的酶谱能较好地区分6种郁金类药用植物；PPO和COD还能够对种内不同居群的材料进行区分。结论 同种植物不同居群间亲缘关系的远近与地理来源有关；能够通过聚类分析区分姜黄和川郁金；姜黄属郁金类植物的栽培型和野生型产生了分化。

Objective To explore the interspecific and intraspecific genetic relationships of six species in Curcuma L. Methods The superoxide dismutase (SOD), polyphenol oxidase (PPO), and cytochrome-oxidase (COD) were studied by vertical slab polyacrylamide gel electrophoresis (PAGE). The three isozymes data were analysed by using clustering method UPGMA. Results The six species in Curcuma L. can largely be distinguished by zymogram of three isozymes; Moreover, PPO and COD can be used to distinguish the different accessions in the same species of Curcuma L. Conclusion The genetic relationships among different accessions are related with the geographical origin. Clustering approach could make a distinction between Curcuma longa and C. sichuanensis. The differentiation has happened between the wild and cultivated species of Curcuma L.

四川省青年科技基金项目（07JQ0085）；留学人员科技活动择优资助项目（川人社函【2010】32号）