目的 研究杜仲多糖对CCl₄致肝纤维化大鼠的保护作用，并探讨其作用机制。方法 采用大鼠首次背部sc纯CCl₄ 5 mL/kg，以后sc 40% CCl₄花生油3 mL/kg，共8周，制备大鼠肝纤维化模型。杜仲多糖ig给药8周，测定大鼠肝脏及脾脏指数，检测大鼠血清中丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)的活性及总蛋白（TP）、白蛋白(ALB)水平，计算ALB与球蛋白（GLOB）的比值（A/G）；检测血清中透明质酸（HA）、层粘连蛋白（LN）、III型前胶原（PCIII）、IV型胶原（IV-C）水平；检测肝脏组织中超氧化物歧化酶（SOD）的活性、羟脯氨酸（Hyp）、丙二醛（MDA）和谷胱甘肽过氧化酶（GSH-Px）的水平；并检测肝脏中转化生长因子β1（TGF-β1）的表达。结果 杜仲多糖能明显对抗CCl₄所致的肝纤维化大鼠肝脏、脾脏指数的升高(P＜0.01）；显著抑制血清中ALT、AST活性的升高（P＜0.01），降低血清中HA、LN、PCIII、IV-C、GLOB的量（P＜0.01），升高血清中的TP、ALB的量和A/G的值（P＜0.01）；降低肝组织中MDA的水平和Hyp的量（P＜0.01），亦能升高肝组织中SOD的活性和GSH-Px的水平（P＜0.01），明显降低肝组织中TGF-β1的表达。杜仲多糖高剂量组抗肝纤维化的效果最好，并呈现明显的剂量依赖性。结论 杜仲多糖具有显著的抗肝纤维化作用。

Objective To study the protective effect of Eucommia ulmoides polysaccharide (EUP) on liver-fibrosis rats and investigate its mechanism. Methods Models of liver-fibrosis rats induced by carbon tetrachlotide (CCl₄) were established by sc injection of pure CCl₄ (5 mL/kg) and then peanut oil with 40% CCl₄ (3 mL/kg) to the back of rats for eight weeks. After the models were ig administrated by EUP for eight weeks, the indexes of the liver and spleen in liver-fibrosis rats were calculated; ALT and AST activities and contents of TP and ALB in serum were examined; Meanwhile, the ratio between ALB and GLOB (A/G) was computed; The four levels of HA, LN, PCIII, and IV-C in serum of liver-fibrosis rats were determined. SOD activities and Hyp, MDA, and GSH-Px levels in liver tissue were examined; The expression of transforming growth factor-β1 (TGF-β1) in liver was observed. Results EUP could obviously be against the index increasing of the liver and spleen (P<0.01) in liver-fibrosis rats induced by CCl₄; remarkably inhibit the increasing of ALT and AST activities in serum (P<0.01); decrease the content of HA, LN, PCIII, IV-C, and GLOB in serum (P<0.01); increase the content of TP and ALB, and ratio of A/G in serum (P<0.01); decrease the MDA amd Hyp levels in liver tissue (P<0.01); and improve SOD activities and GSH-Px levels in liver tissue (P<0.01); and decrease the expression of TGF-β1 as well. The best effect of EUP was observed in the high-dose group and inhibition of EUP on fibrosis was in a dose-dependent manner. Conclusion EUP has the remarkable protective effects on liver-fibrosis rats.

河北省唐山市科技局科研项目（09130223b）