目的 建立同时测定侧柏炭中杨梅素、槲皮苷、槲皮素、山柰酚和穗花杉双黄酮5种黄酮类化合物的超高效液相色谱（UPLC）分析方法。方法 采用Waters Acquity UPLC系统，Waters BEH C₁₈柱（50 mm×2.1 mm，1.7 mm）；流动相为0.05%甲酸水溶液-甲醇，梯度洗脱；体积流量0.2 mL/min；柱温30 ℃；进样量2 μL；检测波长360 nm。结果 杨梅素、槲皮苷、槲皮素、山柰酚和穗花杉双黄酮在线性范围内均具有良好的线性关系（r≥0.999 5）；平均回收率在96.85%～99.01%，RSD≤4.00%。结论 UPLC分离效果及重复性好，且快速、简便，可作为侧柏炭的质量控制方法。

Objective To establish a UPLC method for simultaneous determination of five flavonoids (myricetin, quercitrin, quercetin, kaempferol, and amentoflavone) in Cacumen Platycladi Carbonisatum. Methods The analysis was performed on a Waters Acquity UPLC system with a Waters BEH C₁₈ column (50 mm×2.1 mm, 1.7 mm). The five flavonoids were separated with gradient mobile phase consisting of 0.05% aqueous formic acid and methanol. The temperature of column was 30 ℃, and the injection volume was 2 μL/min, detection wavelength was 360 nm. Results The five flavonoids including myricetin, quercitrin, quercetin, kaempferol, and amentoflavone had good linearity (r≥0.999 5) within the linear ranges. The average recovery rate was 96.85% — 99.01% with RSD≤4.00%. Conclusion The developed UPLC method is simple, sensitive and accurate and has the good repeatability in separation, which is available for the quality control of Cacumen Platycladi Carbonisatum.

国家“十一五”科技攻关项目（2006BAI09B06-02）；中国药典2010年版项目（YS-128）