目的 研究冬青科冬青属植物枸骨Ilex cornuta根中的化学成分。方法 运用硅胶、Sephadex LH-20柱色谱对枸骨根中的化学成分进行分离，通过理化常数、质谱和核磁技术鉴定化合物。结果 从枸骨根中分离并鉴定了11个化合物，分别为β-谷甾醇（1）、羽扇豆醇（2）、白桦酸（3）、常春藤皂苷元（4）、3β-乙酰基-28-羟基-乌苏醇（5）、乌苏酸（6）、19α-羟基乌苏酸（7）、3β-乙酰基-乌苏酸（8）、23-羟基-乌苏甲酯（9）、庚酸（10）、β-胡萝卜苷（11）。结论 化合物4、5、8、9为首次从该属植物中分离得到。化合物3为首次从该植物中分离得到。

ObjectiveTo study the chemical constituents of the roots of Ilex cornuta. Methods The compounds were isolated and purified by silica gel, Sephadex LH-20, medium pressure column chromatography and semi-preparative liquid chromatography and their structures were elucidated by chemical properties and spectroscop analyses. Results Eight compounds were isolated and their structures were identified to be β-sitosterol (1), lupeol (2), betulonic acid (3), hede-ragenin (4), 3β-acetoxy-28-hydroxyurs-12-ene (5), ursolic acid (6), 19α-hydroxy ursolic acid (7), 3β-acetoxy-ursolic acid (8), 23-hydroxyl-methyl ursolate (9), heptanoic acid (10), β-daucosterol (11). Conclusion Compounds 4, 5, 8, and 9 are obtained from this genus for the first time, and compound 3 is obtained from this plant for the first time.

苏州大学青年基金项目（2008）；苏州大学大学生创新试验计划项目资助（2009DC03092）