目的对从野生甘草中选育出的新品种甘草(乌新Ⅰ号)进行种质评价。方法观察了其发芽率、可溶性蛋白量和过氧化物酶活性, 并与对照组进行了基因组多态性比较。结果乌新Ⅰ号可溶性蛋白量产生了变化, 过氧化物酶活性显示乌新Ⅰ号具有较高活性。通过分子标记简单序列重复技术(ISSR)进行多态性分析, 在所使用的22条随机引物中, 6条随机引物产生了不同DNA条带, 表明乌新Ⅰ号甘草与对照组相比产生了基因多态性变化。结论这些变化表明乌新Ⅰ号是一个具有前景的品种并且能在将来广泛应用。

Objective The population structure and ecological distribution of endophytic fungi in Changium smyrnioides from the different habitats and growing phases,and the effects of fungal elicitor on the cell biomass and polysaccharide accumulation were studied in this paper.Methods The isolation,culture,and identification of microorganism,and plant cell suspension culture technology were adopted;And relative data were analyzed by the statistical methods.Results In four producing areas,116 strains were isolated and classified into eight genera.The dominant populations were Fusarium LK.ex FR.,Geotrichum LK.,and Alternaria Nees.The population structure of endophytic fungi obviously changed at the different growing phases.Species and quantity of endophytic fungi were plentiful at the seedling stage and bud stage,and especially at the bud stage the isolation rate and isolation frequency were more than 30% and 19%,respectively.Some endophytic fungi had the obvious area and tissue specificity.Compared with the control by adding the elicitor of Fusarium sp.3,the yields of cell biomass and polysaccharide were increased to 31.86% and 38.01%,respectively.Conclusion Endophytic fungi in C.smyrnioides have abundant biodiversity.And there is close relationship between the population structure and distribution of endophytic fungi with ecological conditions.And fungal elicitors could obviously enhance the yields of cell biomass and polysaccharide of C.smyrnioides.

国家自然科学基金资助项目 (30472148 ,30772732)