目的研究双氢青蒿素对前列腺癌PC-3细胞的凋亡诱导作用, 并探讨其可能的机制。方法MTT法测定不同浓度双氢青蒿素对PC-3细胞增殖的影响, 流式细胞仪(FCM)、透射电镜(TEM)观察双氢青蒿素对PC-3细胞的凋亡诱导作用, 免疫组化(SP)检测双氢青蒿素对PC-细胞内Bax、Bcl-2蛋白阳性表达的影响。结果双氢青蒿素对PC-3细胞有明显增殖抑制效应, 能诱导PC-3细胞凋亡且具有浓度-时间依赖性, 导致PC-3细胞线粒体肿胀、核碎裂、凋亡小体形成;随着双氢青蒿素浓度的增加, Bcl-2蛋白阳性表达降低, 而Bax蛋白阳性表达增加。结论双氢青蒿素可能通过上调Bax, 下调Bcl-2蛋白表达诱导前列腺癌PC-3细胞凋亡。

Objective To study the induction of dihydroartemisinin(DHA) on prostate cancer PC-3 apoptosis and its possible mechansim.Methods MTT was employed for cellular viability measurement,flow cytometry(FCM) and transmission electron microscopy(TEM) for observation of apoptosis,and immunocytochemical staining(SP) for analyzing the expression of Bcl-2 and Bax proteins in PC-3 cells treated with DHA of different concentration.Results DHA Significantly inhibited the proliferation of PC-3 cells,induced their apotosis in a time-concentration dependent manner,and led to mitochondrial swelling,nuclear fragmentations and apoptosis body formation,down-expression of Bcl-2 protein,and over-expression of Bax protein correspondence with DHA concentration.Conclusion DHA could induce the apoptosis in PC-3 cells by up-regulating Bax protein and down-regulating Bcl-2 protein