目的研究抗肿瘤中药青龙衣的化学成分。方法近年大量的药理实验及临床研究表明青龙衣CHCl及EtOAc部分具有显著的抗肿瘤作用, 且毒性较小。本实验利用硅胶柱色谱和大孔吸附树脂分离化合物, 并利用D-NMR及D-NMR光谱数据确定了化合物的结构。结果从青龙衣的CHCl活性部位中分得8个化合物, 并分别确定为0(S)-原人参二醇--酮(1)、达玛烷-20,24-二烯-β-醇(2)、茸毛香杨梅酮(galeon)(3)、胡桃宁A(jugla-nin A)(4)、α, β, -三羟基--烯-8-齐墩果酸(5)、α, β--三羟基--烯-8-熊果酸(6)、齐墩果酸(7)、熊果酸(8)。结论化合物为首次从天然资源中分离得到的新天然产物;化合物及5~8均为首次从胡桃属植物中分离得到, 此前未见该属三萜类化合物的报道;化合物为首次从青龙衣中分离得到。

Objective To study the chemical components from the pericarps of Juglans mandshurica with anti-tumor activity.MethodsIt showed that pericarps of J.mandshurica had a good performance in treating cancer and low toxicity from CHCl3 and EtOAc portions by a lot of pharmacological experiments and modern clinical application in the past.Compounds were isolated by chromatography on silica gel columns and AB-8 macroporous resin.On the basis of spectral evidences including 1D-NMR and 2D-NMR,the structures of these compounds were determined.Results These compounds from CHCl3 portion were identified as 20(S)-protopanaxadiol-3-one(1),dammara-20,24-dien-3β-ol(2),galeon(3),juglanin A(4),2α,3β,23-trihydroxy olean-12-en-28-oic acid(5),2α,3β,23-trihydroxy urs-12-en-28-oic acid(6),oleanolic acid(7),ursolic acid(8).Conclusion Compound 1 is a new natural product.Compounds 2 and 5-8 are isolated firstly from the plants of Juglans L.which are due to pentacyclic triterpane.Compound 3 is isolated from the pericarps of J.mandshurica for the first time.

黑龙江省研究生创新科研项目(YJSCX2007 20177HLJ)