目的研究人参皂苷元衍生物及其抗HL-60肿瘤细胞活性。方法利用Smith降解处理人参总皂苷, 采用硅胶柱色谱、制备型高效液相色谱技术分离、纯化人参皂苷元等产物。通过波谱数据分析产物结构。采用MTT法测试产物抗HL-60肿瘤细胞活性。结果分离鉴定了原人参二醇(PD)、原人参三醇(PT)和4, 5-烯-β, 6α-二羟基-12,20-(1'-羟基)双氧乙基-达玛烷(1), 化合物为新化合物命名为'-羟基双氧乙基原人参三醇。结论化合物为新型人参皂苷元衍生物, 其抑制HL-60细胞增殖的作用强于PD和PT。

Objective To investigate the derivatives of ginsenoside sapogenins and their activities against HL-60 cells.Methods The total ginsenoside extract was treated by Smith degradation.All products were isolated by silica gel chromatography and purified by preparative HPLC.On the basis of 1D and 2D NMR data,structures were elucidated.The activity against HL-60 cells was measured by MTT method.Results Three compounds were isolated and identified as 20(S)-protopanaxadiol(PD),20(S)-protopanaxatriol(PT),and 24,25-en-3β,6α-dihydroxy-12,20-(1'-hydroxy) ethanedioxy-dammarane(1).Conclusion Compound 1,named 1'-hydroxy ethanedioxy PT,is a novel derivate of sapogenin with higher inhibitory activity against HL-60 cells than PD and PT.

教育部博士点基金课题 (20040001136)