目的研究从独一味中分离制备连翘酯苷B和洋丁香苷对照品的方法。方法结合大孔吸附树脂、葡聚糖凝胶、制备HPLC法分离制备独一味中连翘酯苷B和洋丁香苷单体。结果分离制备的连翘酯苷B的质量分数达98.93%,洋丁香苷的质量分数达99.91%。结论该方法所得洋丁香苷和连翘酯苷B质量分数高,可用于制备中药定性、定量分析使用的对照品。

Objective To establish a method for the isolation and preparation of forsythoside B and ac-teoside reference substances from Lamiophlomis rotata.Methods Forsythoside B and acteoside in L. ro-tata were isolated and purified by macroporous resin，Sephadex column，and preparative HPLC. ResultsAnalysis with HPLC showed the content of the prepared acteoside and forsythoside B reached to 98. 93%and 99. 91%，respectively. Conclusion This method is effective for the high purity of prepared acteosideand forsythoside B. It can be used as reference substances for the qualitative and quantitative analyses ofChinese herbal medicine.

“十一五”国家科技支撑计划资助项目(2006BAI06A01-02;2007BAI41B06)