目的从生物物理和生物化学的角度,探讨黄连与吴茱萸分煎后配伍时的药性差异。方法黄连和吴茱萸药材用水分别煎煮,分煎液按相应的比例配伍,在100℃下水浴加热1 h,得单味药分煎后配伍所得的左金丸、甘露散、茱萸丸和反左金丸药液。采用微量热法,测定了痢疾杆菌在类方水提液作用下的生长代谢热谱曲线,获得相应的生物热动力学参数,结合文献报道,综合分析黄连与吴茱萸分煎后配伍时的药性差异。结果黄连与吴茱萸分煎后配伍的药液之间存在稳定的药性差异。左金丸及类方均能不同程度地抑制痢疾杆菌的生长代谢过程,左金丸的抑制作用最强,反左金丸的抑制作用最弱,4方抑菌作用强弱顺序为:左金丸>甘露散>茱萸丸>反左金丸。结论微量热法可用于刻画黄连与吴茱萸分煎后配伍时的差异。

Objective To study drug property differences during combination of Rhixoma Coptidis withFructus Evodiae after separated decoction from biophysics and biochemistry. Methods Rhizoma Coptidisand Fructus Evodiae were decocted with water separately. Different proportions of Rhixoma Coptidisaqueous extract and Fructus Evodiae aqueous extract were combined and heated in an aqueous bath for 1 hat 100 0C to get the decoctions of Zuojin Pill，Ganlu Powder, Zhuyu Pill，and Transzuojin Pill. Micro-calorimetry was used to obtain the metabolic thermal spectrum curves and biothermo-kinetic parameters of Shigella dysenteriae growth. According to the ancient herbal literatures，the drug property differences during combination of Rhixoma Coptidis with Fructus Evodiae after separated decoction were syntheticallyevaluated. Results There was a stable drug property difference between separated decoctions. Both thedecoction of Zuojin Pill and its similar prescriptions could inhibit the growth and metabolism of the bacteriaat different levels. Zuojin Pill had the strongest inhibitory effect，while Transzuojin Pill had the poorest in-hibitory effect on S. dysenteriae growth. The sequence of antibacterial activity was:Zuojin Pill>GanluPowder>Zhuyu Pill>Transzuojin Pill. Conclusion Microcalorimetry can be used to investigate thedrug property differences during combination of Rhizoma Coptidis with Fructus Evodiae after separated decoction.

国家重点基础研究发展计划(973计划)(2007CB512607);国家自然科学基金资助项目(30772740);军队“十一五”自然基金(06MA360)