目的：研究北柴胡叶片、茎段和花芽愈伤组织诱导、分化和不定芽增殖的条件, 探讨快速繁殖的新途径。方法：选择优良的北柴胡类型, 在附加不同种类、不同质量浓度和不同配比外源植物激素的MS培养基中, 进行不同外植体愈伤组织的诱导和分化培养以及愈伤组织再生植株的繁殖和生根培养。结果：附加2, 4-D 1.0 mg/L、KT0.5 mg/L和6-BA 0.5 mg/L的MS培养基最适合于叶片、茎和花芽愈伤组织的诱导, 其中以花芽愈伤组织的诱导效果最好;在附加6-BA 1.0 mg/L、NAA 0.03 mg/L和CM 15%、CH500 mg/L的培养基中, 愈伤组织的分化率最高;附加6-BA 1.5 mg/L、NAA 0. 05 mg/L和CH 250 mg/L的MS培养基为芽苗增殖的适宜培养基;附加NAA0.5 mg/L的1/2 MS培养基是生根的适宜培养基。结论：北柴胡的茎段和花芽外植体在适宜的激素组合中均可通过愈伤组织途径分化出不定芽, 继而得到正常生长、发育的再生植株。

Objective: To study the condition for induction and differentiation of callus and propagation of adventitious buds in lamina, stem, and bud of Bupleurum chinese and establish a new method for rapid propagation. Methods: In MS media added with different phytohormones, calli were induced from explants of lamina, stem, and floral bud of B. chinese, adventitious buds and adventitious roots were differentiated from calli of stern and floral bud。test-tube plantlets were formed. Results: MS Medium added with 2, 4-D 1.0 mg/L, KT0.5 mg/L, and6-BA 0.5 mg/L was suitable for calli induction of the lamina, stems, and floral buds. In medium added with 6-BA 1.0 mg/L, NAA 0.03 mg/L, CM 15% and CH 500mg/L, the differentiation rate of floral buds callus was the highest. MSMedium added with 6-BA 1.5mg/L, NAA 0.05mg/Land CH 250mg/L was suitable for propagation of test-tube plantlets, 1/2MS medium added with NAA 0.5 mg/Lwas suitable for rooting. Conclusion: A greatdeal of test-tube plantlets could be differentiated and propagated rapidly by calli induced from stems and floral buds of B. chinese. Then the regeneration plantlets with normal growth and developmentare obtained.

山西省科技攻关项目(051077-2)