目的：研究芦荟大黄素对植物血凝素(PHA)引起的大鼠T淋巴细胞增殖和细胞内Ca²⁺浓度([Ca²⁺]_i)变化的影响, 探讨芦荟大黄素对T淋巴细胞增殖的作用和机制。方法：采用MTT法检测细胞增殖, 用单细胞钙成像系统记录细胞胞浆[Ca²⁺]_i。结果：芦荟大黄素对PHA引起的细胞增殖有显著抑制作用, 且其抑制作用与剂量有关;加入PHA引起细胞[Ca²⁺]_i迅速升高, 并持续维持高[Ca²⁺]_i水平, 芦荟大黄素对PHA引起的[Ca²⁺]_i升高有显著抑制作用, 其作用途径包括阻断胞内Ca²⁺释放和抑制胞外Ca²⁺内流。结论：芦荟大黄素可抑制T淋巴细胞增殖, 其作用机制可能与抑制淋巴细胞内[Ca²⁺]_i升高相关。

Objective: To investigate the effects of aloe-emodin on PHA-induced proliferation and intracellular Ca²⁺ mobilization of Tlymphocytes. Methods: The proliferation of T cell was tested bv MTT assay. The [Ca²⁺]_i mobilization was recorded by singlecell Ca²⁺ image system using Fura-2 as Ca²⁺ probe. Results: Aloe-emodin inhibited PHA-induced proliferation in a dose-dependent manner. PHA triggered a rapid [Ca²⁺]_i rise followed by a sustainhigh [Ca²⁺]_i level. The rapid Ca²⁺ rise was caused by Ca²⁺ release from intracellular store and the sustain high [Ca²⁺]_i level was due to Ca²⁺ influx. Aloe-emodin inhibited both Ca²⁺ release and Ca²⁺ influx. Conclusion: Aloe-emodin blocks PHA-induced proliferation of T lymphocytesand the mechanism for this maybe due to its inhibition on PHA-induced [Ca²⁺]_i mobilization.

天津市科委重点科技攻关项目(983113411)