目的：研究地黄寡糖改善HepG2细胞胰岛素抵抗的分子机制。方法：运用RT-PCR技术, 检测地黄寡糖对胰岛素抵抗HepG2细胞内过氧化物酶体增殖物激活受体α(PPAR-α)、胰岛素受体(IR)、葡萄糖转运蛋白4(GLUT4)和2(GLUT2)基因表达的影响。结果：地黄寡糖能够促进胰岛素抵抗HepG2细胞中PPAR-α、IR、GLUT4 mRNA的表达;能够明显降低GLUT2基因mRNA的表达。结论：地黄寡糖对HepG2胰岛素抵抗具有明显的改善作用, 其机制可能与激活PPAR-α、调节HepG2内IR及GLUT2 mRNA的表达有关。

Objective: To study the molecular mechanism of Rehmannia glutinosa oligosaccharides (ROS) on the improvement of insulin resistance of HepG2 cell in vitro. Methods: The mRNA expression of peroxisome proliferator-activated receptor alpha(PPAR-a), insulin receptor(IR), GLUT4 and GLUT2 related to insulin sensitivity was detected by reverse transcription PCR(RT-PCR). Results: The mRNA expression of PPAR-a, IR, and GLUT4 was up-regulated by ROS(10mg/L), but the mRNA expression of GLUT2 was inhibited. Conclusion: The improving effect of ROS on the improvement of insulinresistance of HepG2 cell in vitro is related to the activation of PPAR-a and there gulation of mRNA gene expression of IR and GLUT4. as well as the inhibition of GLUT2 gene expression.

国家自然科学基金资助项目(30672643, 30772073);甘肃省自然科学基金资助项目(3ZS051-A25-078)