目的：利用紫外分光光度法、红外分光光度法及核磁共振法研究β-环糊精对异穿心莲内酯的包合作用。方法：采用微波辅助饱和水溶液法制备异穿心莲内酯-β-环糊精包合物, 紫外分光光度法测定包合物的包合常数, 并确定异穿心莲内酯-β-环糊精的包合比例, 红外分光光度法和核磁共振法确证包合物的形成且验证包合比例。结果：异穿心莲内酯与β-环糊精形成了包合物, 包合常数为140. 845 L/mol, 包合比例为1:1。结论：β-环糊精可以包合异穿心莲内酯, 且包合效果较好。

Objective: To study the inclusion effect of isoandrographo1ide-β-cyclodextrin by method of UV, IR, and ¹H-NMR. Methods: The inclusion compound of isoand rographoIide-β-cyclodextrin was prepared by saturated solution method assist microwave, equilibrium constants of inclusion complexes and molar ratio of isoandrogapho1ide-β-cyclodextrin were determined by the UV. The inclusion compound and their molar ratio were confirmed by IR and ¹H-NMR. Results: The isoandrographolide and β-cyclodextrin formed inclusion compound. The equilibrium constants of inclusion complexes were 140.845 L/mol and the molar ratio was 1：1. Conclusion: The isoandrographolide could be inclusedby β-cyclodextrin and the inclusion effect iS better.