目的：研究酶在丹参提取过程中的作用, 确定不同因素对酶解提取效果的影响。方法：采用单因素实验方法与紫外检测分析方法, 以丹参素提取率为评价指标, 对原药粒度、酶用量、固液比、第-步酶解时间及第二步水提时间5个工艺参数分别进行实验, 以确定酶法提取的最佳工艺条件。结果：酶法提取丹参素的最佳工艺条件为:酶解温度50 ℃;pH=4. 6;粒度为20~35目;酶底物质量比1:200;第-步酶解时间为80 min, 固液比为1:15;第二步水提时间为40 min, 固液比为1:9。结论：通过本工艺得到的丹参素提取率为84. 61%, 且工艺条件温和, 对设备要求低, 不仅可以降低能耗, 还可以避免多糖类物质的糊化, 并减小丹参素在提取过程中被氧化的可能。

Objective: To study the effect of enzyme in the extraction of Salvia mitiorrhiza and the influence of different factors on enzymatic extraction. Methods: Monofactorial experiments were employed to examine the effects of the substrate size, enzyme consumption, ratio of solid to liquid, 1^st enzymatic extraction time. and 2^nd water extraction time on the extraction of S. mitiorrhiza. Ultraviolet-visible spectrophocometry was used to analyzethe results and Danshensu extraction rate wasregarded as standard. Results: The data showed that the highest Danshensu extraction rate(84. 61%)could be achieved at：t= 50 ℃, pH=4. 6, granularity=20-35 meshes, E-S=1：200, 1^st enzymaticextraction time=80 min, S-L=1：15, 2^nd water extraction time=40 min, S-L=1：9. Conclusion: Thisprocesscould be conducted under mild conditions and in relatively simple equipment, which could help reduce energy cost, alleviate gelatinization, and lower the possibility of product oxidation, and thus it is considerably feasible.

天津市应用基础研究计划面上项目(05Y[JMJC09500)