目的
研究离体条件下射干试管根茎诱导的最适培养基。
方法
利用植物组织培养技术,研究了碳源种类和质量浓度,NAA质量浓度和活性炭对射干试管根茎诱导的影响。
结果
射干试管根茎诱导的最佳培养基是MS+6-BA2.0mg/L+NAA0.5mg/L+白糖6%,培养基中不宜添加活性炭。试管根茎接入培养基MS+BA2.0mg/L+白糖3%上,发芽率达61.03%。
结论
糖质量浓度是影响射干试管根茎诱导的主要因素。

Objective To optimize the medium for rhizomatous induction of Belamcanda chinensis in vitro.Methods By plant tissue culture technology,the effects of various carbon source,N AA,and activecarbon at different concentration on the rhizomatous formation of B.chinensis in vitro were studied.Results MS+6一BA 2.0 mg／L+NAA 0.5 mg／L+6％white sugar was the optimal medium for the rhiz-omatous formation of B.chinensis in vitro.Active carbon should not be added to the medium.Thegermination rate of rhizomatous in vitro was 61.03％on the MS+BA 2.0 mg／L+3％white sugar.Conclusion Sugar concentration iS the main factor of the influence on the rhizomatous formation of B.chinensis in vitro.