目的研究假地蓝Crotalaria ferruginea全草的化学成分。方法采用硅胶柱色谱、Sephadex LH-20柱色谱进行成分分离纯化,UV、NMR、MS等方法进行结构鉴定。结果从假地蓝全草的醋酸乙酯萃取部分中分离并鉴定了12个化合物,分别为β-谷甾醇(Ⅰ)、高山金链花素(Ⅱ)、染料木素(Ⅲ)、正四十二烷酸(Ⅳ)、正三十二烷酸(Ⅴ)、正三十一烷醇(Ⅵ)、(2S,3S,4R,12E,2′R)-2-(2′-羟基-二十二碳酰胺基)二十碳-1,3,4-三羟基-12-烯(Ⅶ)、胡萝卜苷(Ⅷ)、白桦脂酸(Ⅸ)、3′,5-dihydroxy-4′-methoxy-2″,2″-dimethylpyrano-(5″,6″,6,7)-isoflavone(Ⅹ)、12-oleanene-3β,22β,24-triol(soyasapogenol B,Ⅺ)、laburnetin[4′,5,7-trihydroxy-6-(2-hydroxy-3-methyl-3-butenyl)

Objective To study the chemical constituents of Crotalaria ferruginea.Methods The compounds were isolated by silica gel and Sephadex LH-20 chromatography and their structures were identified by the spectra evidences of UV,NMR,and MS.Results Twelve compounds were isolated from ethyl acetate extract and their structures were identified as β-sitosterol(Ⅰ),alpinumisoflavone(Ⅱ),genistein(Ⅲ),dotetracontanoic acid(Ⅳ),dotriacontanoic acid(Ⅴ),1-hentriacontanol(Ⅵ),(2S,3S,4R,12E,2'R)-2-(2'-hydroxyl-docosanoylamino) eicosane-1,3,4-trihydroxy-12-ene(Ⅶ),daucosterol(Ⅷ),betulinic acid(Ⅸ),3',5-dihydroxy-4'-methoxy-2″,2″-dimethylpyrano-(5″,6″,6,7)-isoflavone(Ⅹ),12-oleanene-3β,22β,24-triol(soyasapogenol B,Ⅺ) and laburnetin [4',5,7-trihydroxy-6-(2-hydroxy-3-methyl-3-butenyl) isoflavone,ⅩⅡ].Conclusion All these compounds are reported in this plant for the first time.

国家自然科学基金项目(30460150)；国家重大基础研究前期研究专项(“973”前期)(2004CCA03800)