目的研究毛茛Ranunculus japonicus提取物的化学成分。方法采用硅胶和Sephadex LH-20柱色谱进行成分分离,通过光谱分析鉴定所分离成分的结构。结果分离获得一个油状样品。该样品经正、反相薄层色谱均为单一斑点,经GC-MS及反相HPLC柱色谱分析均为单一峰。但ESI-MS和¹D、²DNMR谱学分析确定其为毛茛苷元(ranunculinin,Ⅰ)、异毛茛苷元(isoranunculinin,Ⅱ)和二氢毛茛苷元(dihydroranunculinin,Ⅲ)3个组分以2∶1∶1比率的混合物。结论所有化合物均为从该植物中首次分离得到,其中化合物Ⅱ和Ⅲ为首次从天然中分离得到。

Objective To study the chemical constituents in the extract from Ranunculus japonicus. Methods Silica gel and Sephadex LH-20 open columns chromatography were used to isolate the cons-tituents from 85% alcoholic extract of the title plant. The structures of the isolated constituents were identified on the basis of spectral analyses. Results An oil sample was obtained, which showed a single spot by TLC with variety of the developing solvent systems. The oil was detected as a single peak by both GC-MS and RP-HPLC. However, analystic data of ESI-MS, 1D and 2D NMR revealed that the sample was the mixture of three compounds: ranunculinin (Ⅰ), isoranunculinin (Ⅱ), and dihydroranunculinin (Ⅲ) in the ratio of 2∶1∶1. Conclusion All of three compounds are obtained from the plant for the first time. Among them, compounds Ⅱ and Ⅲ are isolated as the natural products for the first time.

广东科技厅课题(2004B30101002)；广东省中医药管理局课题(1040083)