目的 探讨阿魏酸钠及白芍总苷对小鼠H22肿瘤生长及血管生成的影响。方法 昆明小鼠前腋下皮下接种小鼠H22肝癌细胞,第2天ip阿魏酸钠(200、100、50mg/kg)或ig白芍总苷(200、100、50mg/kg),每3天测量1次肿瘤体积。用免疫组化法标记肿瘤血管及测定血管内皮细胞生长因子(VEGF)及增殖细胞核抗原(PCNA)的表达。MTT法检测阿魏酸钠对H22肿瘤细胞体外增殖的影响。结果 阿魏酸钠组小鼠H22肿瘤体积、质量增长明显较模型组缓慢(P<0.05),同时阿魏酸钠组的肿瘤微血管密度及VEGF、PCNA阳性细胞较模型组显著减少(P<0.05),阿魏酸钠对小鼠H22肿瘤细胞体外增殖无显著抑制作用。白芍总苷各剂量组的肿瘤生长、微血管密度、H22肿瘤细胞增殖与模型组相比均无显著性差异。结论 阿魏酸钠可以显著抑制小鼠H22肿瘤的生长及血管生成,且能抑制VEGF的表达。但在体外实验中不能抑制H22肿瘤细胞的增殖。白芍总苷尚不能被证明有抑制小鼠H22肿瘤生长的作用。阿魏酸钠对肿瘤组织VEGF表达的抑制作用可能是其抗H22肿瘤及抗血管生成的一个重要原因。

Objective? To investigate the effect of sodium ferulate and total glucosides in paeony on tumor growth and angiogenesis in H22 mice. Methods? H22 Cells were inoculated subcutaneously into anteroaxilla of KM mice. Animals were randomized for therapy on the second day and treated with ip sodium ferulate 200, 100, and 50 mg/(kg·d), or ig the total glucosides of paeony 200, 100, and 50 mg/(kg·d). The volume of tumor was measured at three days intervals. The expression of tumor microvessel, VEGF, and PCNA were examined by immunohistochemical staining. The proliferation of H22 cells in virto was examined by MTT assay. Results? In vivo, the increase of tumor volumn and weight in sodium ferulate group was obvious slower than that in model group (P0.05). The treatment with sodium ferulate inhibited the expression of VEGF (P0.05), leading to a decrease in microvessel density, which also decreased the positive cell of VEGF and PCNA (P0.05) within tumor. In vitro, sodium ferulate failed to inhibit the proliferation of H22 cells. In addition, it can not be proved that those in the groups treated with total glucosides in paeony in every dosages are significantly different compared with the model group. Conclusion?? Sodium ferulate inhibits tumor growth, angiogenesis as well as VEGF expression significantly in H22 mice. While total glucosides of paeony can not inhibit the tumor growth, angiogenesis as well as the VEGF expression in H22 mice.

国家自然科学基金资助项目(300070915)