目的 探讨二精丸有效部位对肾阴虚模型大鼠学习记忆障碍的影响及其分子机制。方法 采用大鼠ig甲状腺素加im利血平方法 制备肾阴虚模型,二精丸总黄酮和总多糖ig给药结束后Morris水迷宫法测定各组的学习记忆能力;采用酶联免疫法测定血清激素水平;采用免疫组织化学法测定海马部位Bcl-2和Bax表达及Tau蛋白异常磷酸化水平。结果 二精丸总黄酮和总多糖能显著提高模型大鼠的学习记忆能力;能显著恢复激素水平及海马区Bcl-2和Bax表达;能显著抑制海马区Tau蛋白Ser199/202、Ser396、Thr231三个磷酸化位点异常磷酸化。结论 二精丸总黄酮和总多糖能提高肾阴虚模型大鼠的学习记忆能力。

Objective? To investigate the effect of active fractions in Erjing Pill on learning dysmnesia of rat with kidney-YIN deficiency and its molecular mechanism. Methods? Model rat with kidney-YIN deficiency was made by ig administrating tetraiodothyronine and im injecting Reserpine. Morris water-maze was used to determine the learning and memory ability of every groups after ig administrating total flavones and polysaccharides; enzyme-linked immunoassay (ELIA) was used to determine the level of serum hormone; immunohistochemical method was used to determine the expression of Bcl-2 and Bax and the level of Tau protein abnormality phosphorylation in HIP. Results? Total flavones and polysaccharides in Erjing Pill could obviously elevate the learning and memory ability of model rats, they could obviously recover the level of hormone and the expression of Bcl-2 and Bax in HIP; they cold obviously restrain the abnormality phosphorylation in Tau protein Ser199/202, Ser396, and Thr231 phosphorylation situs in HIP. Conclusion?? The total flavones and polysaccharides in Erjing Pill could promote the learning and memory ability of model rat with kidney-YIN deficiency.

国家自然科学基金资助项目(30360123)