目的研究苦荞茎叶粉中总黄酮酶法提取工艺。方法苦荞茎叶粉经纤维素酶处理后用水提取总黄酮,研究酶加酶量、解温度、酶解时间和pH值对总黄酮得率的影响。结果酶法提取的最佳工艺条件为酶解温度55℃,加酶量3.0μL,pH值6.5,酶解90min,再在90℃下提取3次,每次30min,总黄酮得率可达1.47%。结论纤维素酶适用于苦荞茎叶粉中总黄酮的辅助提取。

ObjectiveTooptimizetheextracting technologyof total flavones inpowderofFagopyrumtataricttm stem and leafby enzymatictreatment．Methods Thepowderwas treatedbycellulase before ex-tractedusingwater，theeffects ofenzyme dosage，treatment temperature，treatmenttime，and pHvalueonthe extract rate of total flavones were studied．Results Theoptimum extracting technologywasasfol-lows：enzymatictreatmenttemperature：55 C，enzyme dosage：3．0 tA，，pHvalue：6．5，treatment time：90min，extracting3 timesat：90C for 30 min once．The extract rate of total flavones was 1．47％by thistechnology．ConclusionCellulase could beappliedin the assisstant extraction of total flavones inpowderof F．tataricum stem and leaf．

科技部攻关计划重大项目(2003BA901A19);西安市2003年农业科技攻关计划项目(NG200317)