[关键词]
[摘要]
目的分离、纯化具有降血糖作用的仙人掌多糖组分。方法经热水提取、乙醇沉淀、DEAE Sepharose fast flow离子交换色谱和Sephadex G系列凝胶滤过色谱纯化得到5种仙人掌多糖组分。醋酸纤维薄膜电泳检测多糖纯度,凝胶色谱测定相对分子质量,高效液相色谱测定糖的组成。结果5种多糖都已达到电泳纯。它们的相对分子质量依次为2.0×103、1.0×106、4.0×103、9.2×105、5.0×103。ODP1由鼠李糖组成,ODP2可能由鼠李糖和葡萄糖组成,ODP4可能由鼠李糖和D-半乳糖组成。ODP3和ODP5分别由鼠李糖和一未知糖组分组成。结论仙人掌多糖的提取没有必要采用脱蛋白、脱色素步骤,本实验所提取的仙人掌多糖是均一的。
[Key word]
[Abstract]
ObjectiveToisolate，purify，and identifytheOpuntiadilleniipolysaccharide(ODP)．MethodsFreshcactus was extractedbyhotwater，precipitated byethanol，fractionated byDEAESepharosefastflow，separated by SephadexG series，and treatedby freeze-drying．Afterthe abovepurifi-cation，fivepolysaccharides were obtained．Purityof them was examinedby electrophoresis，relativemolecularweightof t hem was measuredby gel chromatography，andchemicalcompositionwas determinedbyHPI．C．Results All of ODP wereprovedtobehomogenous，relativemolecularweightof them were2．0×103，1．0×106，4．0×103，9．2×105，and 5．0×103．ODPIwascomposedof rhamnoseonly，ODP2was madeupof rhamnose andD-glucose，ODP4contained rhamnose andD-galaetose，()DP3and ODP5consisted of rhamnose and another unidentifiedmonosaccharide。respectively．ConclusionThedepro-teinizedordecolorizedprocedureiS notnecessaryfor thepurificationof ODP，andallof()DPareidentifiedtObehomogenous．
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[基金项目]
广东省高校自然科学基金资助项目(Z03062);湛江市科技招标计划项目(湛财企[2004]98号)
