目的研究白藜芦醇对人脑胶质瘤细胞U251细胞生长抑制及诱导细胞凋亡的作用。方法采用MTT法检测白藜芦醇对U251细胞的增殖活性的影响,采用流式细胞仪检测白藜芦醇对U251细胞早期、晚期凋亡率的影响,用倒置显微镜观察不同浓度的白藜芦醇作用后U251细胞的形态学改变。结果白藜芦醇对U251脑胶质瘤生长有抑制作用,且具时间及剂量依赖性;白藜芦醇能诱导U251细胞早、晚期凋亡,且具时间及剂量依赖性。结论白藜芦醇能抑制U251脑胶质瘤的增殖并能诱导细胞凋亡。

Objective To investigate the effects of resveratrol on inhibiting proliferation and inducing apoptosis of U251 cells. Methods The proliferation activity was detected by MTT assay, and apoptosis of early and late period by method of Annexin V-FITC apoptosis assay, and the cell morphological changes were observed by microscopy after U251 cells being treated with resveratrol. Results Resveratrol could significantly inhibite the proliferation and induce the apoptosis of early and late period of U251 cell in a time-and does-dependent manner. Conclusion Resveratrol can significantly inhibite the proliferation and induce the apoptosis of early and late period of U251 cell.