目的研究当归A₃活性部位(A₃)的抗炎作用及其对脂多糖(LPS)诱导离体大鼠子宫环氧化酶-2(Cox-2)表达增高的影响。方法采用二甲苯致小鼠耳廓肿胀和角叉菜胶致大鼠足趾肿胀法进行抗炎药效学实验;采用RT-PCR和Westernblotting法检测Cox-2mRNA及蛋白表达水平。结果A₃(1、5、10mg/kg)可剂量依赖性地抑制二甲苯所致的小鼠耳廓肿胀和角叉菜胶所致的大鼠足趾肿胀。LPS1μg/mL可显著增加离体大鼠子宫Cox-2mRNA和蛋白表达水平。A₃(10~320mg/L)剂量依赖性地抑制LPS诱导的子宫Cox-2mRNA和蛋白表达水平增高。结论A₃具有较强的抗炎作用,其抗炎作用机制可能与抑制Cox-2mRNA及蛋白表达有关。

Objective To study the inhibitory effects of angelica A₃ active fraction (A₃) inflammation and up-regulation of cyclooxygenase-2 (Cox-2) expression of rat uterus induced by lipopolysaccharides (LPS). Methods The anti-inflammatory effects of A₃ were investigated in rats using the carrageenin-induced paw swelling model and in mice using dimethylbenzene-induced ear edema model; RT-PCR and Western blotting were used to analyze Cox-2 mRNA and the protein expression levels. Results A₃ (1, 5, and 10 mg/kg) dose-dependently inhibited dimethylbenzene-induced ear edema in mice and paw swelling in rats by ig administration. LPS 1 μg/mL could significantly increase the level of Cox-2 mRNA and protein expression. A₃(10—320 mg/L) could concentration-dependently inhibit Cox-2 mRNA and protein over-expression stimulated by LPS. Conclusion A₃ possesses better anti-inflammatory effects than angelica oil, which maybe relates to its inhibitory effects on Cox-2 overexpression.

国家自然科学基金资助项目(30171095)