目的探讨芪丹通脉片(QDTM T)对急性心肌缺血的防治作用。方法SD大鼠ig QDTM T(2 g/kg)两周后,sc异丙肾上腺素(Iso,5 m g/kg)诱导急性心肌缺血模型。通过透射电镜观察心肌超微结构的变化;用比色法测定心肌组织及心肌细胞线粒体中C a2+水平及心肌细胞线粒体中C a2+-ATPase活力的变化。结果大鼠igQDTM T两周后,sc Iso所造成的心肌超微结构的损害明显减轻;心肌细胞线粒体中C a2+-ATPase活力增加,而心肌组织及心肌细胞线粒体中C a2+水平显著降低(P<0.01)。结论QDTM T可能是通过抑制心肌细胞线粒体中钙超载及保护心肌细胞超微结构,达到防治急性心肌缺血的目的。

Objective To study the protective effect of Qidan Tongmai Tablet (QDTMT) on the (injury) induced by acute myocardial ischemia. Methods Acute myocardial ischemia was induced by sc isoproterenol (Iso, 5 mg/kg) into SD rats pretreated with ig QDTMT (2 g/kg) for two weeks. Activities of Ca~(2+)-(ATPase) in myocardial mitochondria and content of Ca~(2+) in myocardium and myocardial mitochondria were determined by colorimetric method. The changes of myocardial ultrastructure were investigated by means of transmission electron microscope. Results Compared with the controls, activities of Ca~(2+)-(ATPase) in mitochondrion were strengthened in QDTMT group and the content of Ca~(2+) was decreased ((P0.01)) in myocardium and myocardial mitochondrion while myocardial ultrastructure damage were significantly reduced in QDTMT group. Conclusion QDTMT can relieve calcium overload in cardiac mitochondrion and protect the integrity of the ultrastructure of cardiac, and thus be used in prophylaxis and treatment of acute myocardial ischemia.

陕西省攻关课题(2001,12K-G7)