目的研究补阳还五汤对血瘀证大鼠血管内皮细胞黏附分子表达的影响。方法采用免疫组织化学和RT-PCR方法观察补阳还五汤不同剂量对血瘀证大鼠血管内皮细胞细胞间黏附分子-1(ICAM-1)、血管细胞黏附分子-1(VCAM-1)、血小板-内皮细胞黏附分子-1(PECAM-1)和诱生型一氧化氮合酶(iNO S)表达的影响。结果模型组ICAM-1、VCAM-1、PECAM-1、iNO S高表达,补阳还五汤能减少造模动物ICAM-1、VCAM-1、PECAM-1、iNO S的表达,而且随着药物剂量的减少,各分子表达呈递增趋势,具有明显的量效关系。结论补阳还五汤能显著降低血瘀证大鼠血管内皮细胞黏附分子高表达,且量效关系明显。

Objective To study the effect of Buyang Huanwu Decoction (BHD) on adhesion molecules in vascular endothelial cells (VECs) of blood stasis rats. Methods Expression of ICAM-1, VCAM-1, PECAM-1, iNOS in vascular endothelial cells of blood stasis rats receiving different doses of BHD was observed by immunohistochemical method and RT-PCR. Results The expression of ICAM-1, VCAM-1, PECAM-1, iNOS was increased in model group, while was decreased by BHD. There was an increase tendency on the expression of molecules and significant dose-effect relationship along with the decrease of the BHD doses. Conclusion The increased expression of adhesion molecules in vascular endothelial cells of blood stasis rats could be reduced by BHD in a dose dependent manner.

国家自然科学基金资助项目(30070909);广东省“千百十工程”优秀人才培养基金资助项目(Q0262)