目的 观察龙葵碱对H₂₂荷瘤小鼠肿瘤细胞膜流动性和膜蛋白水平的影响。方法 采用荧光探针DPH标记法测定肿瘤细胞膜流动性,考马斯亮蓝法测定肿瘤细胞膜蛋白水平。结果 龙葵碱可显著降低H₂₂荷瘤小鼠肿瘤细胞膜流动性,降低H₂₂小鼠肿瘤细胞膜蛋白水平。结论 龙葵碱是通过影响肿瘤细胞膜的流动性和肿瘤细胞膜上的蛋白水平恢复细胞的正常生理活性而达到抗肿瘤作用。

Objective To study the effects of alkaloid of Solanum nigrum on membrane fluidity and membrane protein level of tumor cells in H₂₂ tumor-bearing mice(H₂₂ mice).Methods The membrane fluidity of tumor cells in H₂₂ mice was observed by DPH fluorescentprobe marker method,and the level of membrane proteins of tumor cells in H₂₂ mice were determined by Coomassie brilliant blue.Results The alkaloid of S.nigrum could make the membrane fluidity decline and the 1evel of membrane proteins of tumor cells in H₂₂ mice decrease.Conclusion The alkaloid of S.nigrum shows the antitumor activity through decreasing the membrane fluidity and the membrane protein level of tumor cells in H₂₂ mice.

国家自然科学基金资助项目(30400591);黑龙江省自然科学基金资助项目(D0024-13);哈尔滨市青年科学基金资助项目(2004AFQXJ035)