目的 研究补阳还五汤(BYHWD)中生物碱、多糖、苷、苷元4类有效部位对大脑中动脉闭塞(MCAO)后局灶性脑缺血大鼠脑梗死的作用。方法 用颈内动脉线栓法建立大鼠局灶性脑缺血模型,检测脑梗死体积,并比较其神经功能行为评分。结果 脑缺血6h,模型组脑梗死体积显著增大(P<0.01),神经功能行为评分显著升高(P<0.01);生物碱、多糖、苷、苷元各组脑梗死体积较模型组缩小(P<0.05、0.01);生物碱、苷、苷元和多糖可使MCAO大鼠神经功能行为评分下降(P<0.05、0.01),其中以生物碱和苷的作用较强。结论 MCAO后,大鼠脑组织出现梗死,补阳还五汤4类有效部位生物碱、多糖、苷、苷元能减少脑梗死体积,改善脑缺血后神经行为症状。生物碱和苷的抗脑缺血作用较强,这可能是补阳还五汤抗缺血性脑损伤的部分物质基础。

Objective To investigate the effect of four kinds of active fractions(alkaloid,polysaccha-ride,glycoside,aglycone)of Buyang Huanwu Decoction(BYHWD)on the volume of cerebral infarction and the scores of nervous function behavior in focal cerebral ischemia rats after middle cerebral artery OC-clusion(MCAO).Methods The model rats of MCAO were induced by the method of nylon monofilament via the internal carotid artery for 6 h。image analysis technique was used to calculate the volume of cerebral infarction and to record the scores of nervous function behavior,then to compare them.Results After cerebral ischemia for 6 h,both the volume of eerebral infarction of rats in the model group and the scores of the nervous function behavior were increased markedly(P<O.01)：the volumes of cerebral infarction of rats in alkaloid,polysaccharide,glycoside,and aglycone groups were significantly lower than that of rats in the model group(P<O.05,0.01);the scores of the nervous function behavior of rats in alkaloid,polysaccharide,glycoside,and aglycone groups were significantly lower than that of rats in the model group(P<0.05,0.01).Effects of alkaloid and glycoside were SUperior to that of polysaccharide and agly-cone.Conclusion After MCAO,the brain tissue occurs infarction.The four kinds of active fractions of BYHWD can decrease the volume of cerebral infarction of rats and improve the nervous function behavior symptom of rats after MCAO.Effects of alkaloid and glycoside are superior to that of polysaccharide and aglycone。which may be the partial substance basis of BYHWD antagonising cerebral ischemia injury.

国家自然科学基金资助项目(30171132);教育部高等学校学术与技术带头人资助项目;湖南省优秀博士论文作者科研项目