目的 探讨复方血尿停治疗以系膜增生为主要病理改变的肾小球疾病的作用机制。方法 将复方血尿停与大鼠肝微粒体共同孵育后加入到肾小球系膜细胞(GMC)体外培养体系中,MTT法观察细胞增殖情况,放射免疫法测定白细胞介素-6(IL-6)、内皮素-1(ET-1)水平,速率法检测乳酸脱氢酶(LDH)的含量。结果 经肝微粒体代谢后,复方血尿停2、4、8mg/mL均抑制GMC的增殖及IL-6、ET-1的产生,且呈剂量依赖方式。结论 复方血尿停能抑制IL-6、ET-1的分泌和GMC的增殖,此作用可能是其治疗系膜增生性肾小球疾病的作用机制之-。

Objective To study the mechanism of traditional Chinese medicine Compound Xueniaoting (CXNT)in treating glomerulus diseases with the main pathological changes of proliferation in mesangial cells.Methods CXNT and rat liver microsomes were incubated together。and then the incubated CXNT was added into cultured glomerular mesangial cells(GMC)in vitro.The proliferation of GMC was observed by MTT assay,the 1evels of interleukin-6(IL-6)and endothelin-1(ET-1)were determined by radioimmunoassay,and content of lactic dehydrogenase(LDH)was determined by velocity assay.Results CXNT(2,4,8 mg/mL)metobolized by rat liver microsomes could all inhibite the proliferation of GMC and production of IL-6 and ET-1 in a dose-dependent manner.Conclusion CXNT can inhibite the prolif-eration of GMC and restrain the secretion of IL-6 and ET-1,this function may be one of C：XNT mecha-nisms in treating some mesangial proliferative glomerulonephritis.

河南省自然科学基金资助项目(0111023200)