目的 明确双黄连粉针剂体内外抗病毒谱及作用机制。方法 采用组织细胞培养法、染料摄入法,检测双黄连粉针剂对不同种病毒的作用,改变给药时间、途径,探讨双黄连粉针剂抗病毒作用环节。建立病毒性心肌炎、胰腺炎、流感动物模型,通过组织病理学检查法,观察双黄连粉针剂对感染动物的保护作用。结果 双黄连粉针剂具有明显抗流感病毒、呼吸道合胞病毒、腺病毒型、单纯疱疹病毒Ⅰ及Ⅱ型、柯萨奇病毒B₃,A₁₆、新型肠道病毒71型的作用。对脊髓灰质炎病毒Ⅲ型、埃可病毒6型、麻疹病毒、水泡性口炎病毒有一定的抑制作用。并能显著抑制肺炎、心肌炎、胰腺炎的发生,疗效与清开灵相似,优于穿琥宁粉针剂、鱼腥草、莪术油注射液。结论 双黄连粉针剂是一个较广谱的抗病毒针剂,抗病毒作用是多途径的。

Object It was to expound antivirus spectrum and the mechanism of antiviral action of SHUANGHUANGLIAN INJECTION (SHLI). Methods To observe the inhibition of various virus by SHLI, the methods of tissue culture, cell culture as well as dye absorption test were used. The antiviral effect of SHLI was studied by using SHLI with different administration procedure and route. The animal models of myocarditis, insulitis and pneumonia were made and the protection of SHLI in these animal models was observed. Results SHLI has significant antivirus effect against influenza virus, measles virus, respiratory syncytial virus, adenovirus, herpes simplex virus and was more excellent than CHUANHUNING, YUXINGCAO, EZHUYOU INJECTIONs. It also can strongly inhibit the development of myocarditis, insulitis and pneumonia, and has a little antivirus effect against enterovirus. Conclusion SHLI is a wide-spectrum antivirus medicine and has various antiviral mechanism.