目的 体外观察164种中药95%乙醇提取物对白色念珠菌、啤酒酵母和威克海姆原藻3种真菌的作用,并对其中抑菌效果明显的中药中的主要成分进行抗真菌药敏试验。方法 半固体药基混合法,微量稀释法。结果 22种中药乙醇提取物对1种或几种真菌显示较强的抑制作用,所试验的单体化合物对3种真菌无显著抑制作用。结论 首次对中药95%乙醇提取物应用半固体药基混合法进行抗真菌活性筛选,土槿皮、牡丹皮95%乙醇粗提物对3种真菌均有较强的抑制作用。

Object To screen antifungal agents against Candida albicans (Robin) Berkh, Saccharomyces cerevisiac Hansen GL-7 and Prototheca wickerhamii from 164 traditional Chinese medicines (TCMs). Methods The extracts of 164 TCMs were prepared with 95% ethanol and the antifungal activity of these extracts against above three fungi was measured by semisolid agar dilution method. Results The ethanol extracts from 22 TCMs exhibited potent antifungal activity. Conclusion The antifunal activity of the ethanol extracts from TCMs was measured by using semisolid agar dilution method for the first time. The ethanol extracts from the bark of Pseudolarix kaempferi Gord. and Paeonia suffruticosa Andr. are more potent antifungal agents than those of tested other medicines.

国家自然科学基金(N0.30070917)